Question

ou sequence many of the recombinant plasmids generated. You find three mutant versions of the inserted gene. (This is likely due to errors introduced during the PCR amplification.) The sequences of the gene found in the three mutants are given below.

Consider each of the following mutations separately.

i) Mutant A: Suppose that the T/A base pair at position 27 and indicated by a line was changed to a G/C base pair. What would the amino acid sequence of the resulting protein be?

Be sure to enter the sequence N- to -C from left to right. Do not include the "N-" or "-C". Please use the 3-letter amino acid abbreviations (for example, "Ala-Gly-Ser"). We will ignore spaces and dashes when we grade your answer.