The question asks which of the listed human actions does not affect the nitrogen cycle. Let's analyze each option:
A) Use of agricultural fertilizers - This definitely affects the nitrogen cycle as fertilizers often contain nitrogen compounds, which can lead to increased nitrogen levels in the soil and affect surrounding ecosystems.
B) Runoff of pesticides into streams and lakes - While this action primarily concerns chemicals that control pests, it doesn't significantly impact the nitrogen cycle directly compared to the others listed. However, it can affect water quality and ecosystems.
C) Production of sewage and animal wastes from farms - This does affect the nitrogen cycle as both sewage and animal waste are sources of nitrogen that can lead to nutrient pollution when they enter waterways.
D) Removal of soil bacteria from irrigation or construction - This action affects the nitrogen cycle because soil bacteria are crucial for processes like nitrogen fixation and nitrification.
Based on this analysis, the answer that least directly affects the nitrogen cycle is:
B) Runoff of pesticides into streams and lakes.