The BEST explanation for the experimental setup in Study 2 is:
A Keeping a sample in the dark provides the researchers with a baseline on the rate of nanoparticle formation.
In this study, by keeping one set of samples in the dark, the researchers can establish a control that helps them understand how much nanoparticle formation occurs without the influence of light. This allows them to compare the results of the light-exposed samples to those kept in the dark, providing a clear baseline for measuring the effect of light on the nanoparticle formation process.