My lab had done protein crystallography with lysozyme. Two solutions were used to prepare the crystallization: lysosome solution and the precipitate solution.
1) There was a distinct amount of starting material in a well. what would that do to the size of the crystals if the crystal number increases? Why? The size will decrease?
2) What would be a negative consequence of thoroughly mixing the lysosome and precipitate solutions? Why? No crystals will appear?
Thank you for the help!