Match the functions listed with the most appropriate ingredient/reagent

Ingredient/Reagent:

Agarose
TAE buffer
DNA ladder
Glycerol in loading buffer
Colored eyes in loading buffer
Safe-Green ^TM in loading buffer

Purpose:

a. allows user to track migration of sample through the gel
b. conducts current through the gel
c. binds to DNA and fluoresces when exposed to ultraviolet light
d. weighs the samples down so that they sink into the wells of the gel
e. the gel matrix
f. to estimate the size of unknown DNA fragments

1 answer

Here are the matches between the ingredients/reagents and their corresponding purposes:

  1. Agarose - e. the gel matrix
  2. TAE buffer - b. conducts current through the gel
  3. DNA ladder - f. to estimate the size of unknown DNA fragments
  4. Glycerol in loading buffer - d. weighs the samples down so that they sink into the wells of the gel
  5. Colored eyes in loading buffer - a. allows user to track migration of sample through the gel
  6. Safe-Green™ in loading buffer - c. binds to DNA and fluoresces when exposed to ultraviolet light