To identify a substitution mutation, we need to compare the original DNA sequence with the options provided and look for a change in a single nucleotide that replaces another.
The original sequences are:
Original Sequence 1:
C G T T A C T G C A A T
Original Sequence 2:
G C A A T G A C G T T A
Now, let's review the options you provided:
-
Option 1: C G T G A C T G C A A T
G C A C T G A C G T T A
(Here, G is substituted for T in sequence 1, and C is substituted for A in sequence 2.) -
Option 2: C G T T A C T G C A A T
G C A A T G A C G T T A
(No mutations - identical to the original) -
Option 3: C G T T A C T G C A A T
G C A A T G A C G T T A
(Identical to the original; thus, no substitutions) -
Option 4: C G T C T A C T G C A A T
G C A G A T G A C G T T A
(C is substituted for T in the first sequence, A is substituted for A, and thus it has substitutions) -
Option 5: C G T A C T G C A A T
G C A T G A C G T T A
(T is substituted for T in the first sequence, making it a substitution in the original sequence and A is substituted for C in the second sequence)
Based on the definitions of substitution mutations:
- Option 4 includes a change from T to C in the first sequence, making this a substitution mutation.
So, Option 4 shows a substitution mutation.
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