To identify a substitution mutation in the provided sequences, we should compare each sequence to the original DNA sequence:
Original sequence:
C G T T A C T G C A A T G C A A T G A C G T T A
Now, let's analyze the provided sequences:
-
C G T T A C T G C A A T G C A A T G A C G T T A
(No mutation, this matches the original sequence.) -
C G T T A C T G C A A T G C A A T G A C G T T A
(No mutation, this matches the original sequence.) -
C G T C T A C T G C A A T G C A G A T G A C G T T A
(Substitution mutation: G is substituted with C in position 3.) -
C G T T A C T G C A A T G C A G A T G A C G T T A
(Substitution mutation: G is substituted with C in position 3, which is the same as sequence 3.) -
C G T G A C T G C A A T G C A C T G A C G T T A
(Substitution mutation: T is substituted with G in position 3.) -
C G T A C T G C A A T G C A T G A C G T T A
(Substitution mutation: T is replaced with A in position 3.)
From the above sequences, the sequence with a substitution mutation is sequences 3, 4, and 5. Specifically, sequence 3 ("C G T C T A C T ...") has a clear substitution mutation in the third position when compared to the original sequence.
So the answer would be:
C G T C T A C T G C A A T G C A G A T G A C G T T A