Is this data about editing beta thalassaemia or about sickell cell diseases? We isolated CD34+ cells from the differentiated cells on day 6 of the OP9 coculture system and further expanded erythroid cells using the three-stage culture system [19]. Upon erythroid culture, both the Eẞ-iPSC2 cells and the corrected C46 cells gradually changed their morphology from that representing proerythroblasts/basophilic erythroblasts on day 13 of culture to that representing polychromatic/orthochromatic erythroblasts on day 23 and finally became orthochromatic erythroblasts/reticulocytes on day 29 of culture. Analysis of gene expression during the erythroid liquid culture demonstrated that both differentiated Eẞ-iPSC2 and the corrected C46 cells at day 19 of differentiation (when the morphological stages are equivalent to those of day 13 erythroid cells derived from peripheral blood progenitors) expressed lower levels of erythroid- associated transcription factors KLF1 and BCL11A as compared to the cultured erythroblasts from peripheral blood CD34+ cells (Fig. 4c

Answer 1

This data is about editing beta thalassaemia, as it discusses the isolation and expansion of erythroid cells from CD34+ cells derived from Eß-iPSC2 and corrected C46 cells. The study also analyzes gene expression during erythroid culture and compares it to erythroid cells derived from peripheral blood progenitors. The focus is on the expression levels of erythroid-associated transcription factors KLF1 and BCL11A.