The settlers of Jamestown were primarily motivated by a few key factors:
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Setting up a protected colony: They chose a location that offered some security from potential threats, notably from Spanish ships.
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Locating gold and other riches: One of their main goals was to find gold or other valuable resources that could generate profits for themselves and the Virginia Company.
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Discovering a water route to Asia: They also hoped to find a waterway that would facilitate trade with Asia.
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Fighting the spread of disease: While the location they chose ended up being unhealthy and contributed to the spread of disease, combating disease was not one of their initial goals when selecting the site for their settlement.
In summary, the primary motivations were setting up a protected colony, locating gold and other riches, and discovering a water route to Asia, while dealing with disease was an unfortunate reality they faced after establishing their settlement.