In a thin layer chromatography (TLC)experiment,the TLC plate was pre-developed in the development chamber containing the mobile phase (to remove any contaminants before use),then air dried.
On the same day,the TLC plate was then spotted with the sample & standard and placed in the same development chamber containing the mobile phase. Is this acceptable or should the development chamber be replaced with fresh mobile phase?
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I don't know the text book answer but I think the mobile phase should be replaced.
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