In a reaction you use 400 ul of 12mM B-galactosidase. Add 2.3 ml of buffer and 0.3ml of ONPG to a cuvette. what is the final concentration of the cuvette? Answer below to one decimal place.

Answer 1

Also...A stock solution of ONPG has a concentration of 25 mM. Add 2.8 mL of buffer, 0.3
mL of enzyme and 0.9 mL of ONPG to a cuvette. What is the final concentration of
ONPG in the cuvette? Answer below to one decimal place

Answer 2

you are diluting it (.4+2.3+.3)/.4=7.5 times
final concentration=12.5/7.5 mM B-galactosidase

second diluting it (2.8+.3+.9)/.9=4.4times
concentration=25/4.4=5.7 mM