In a chromatography experiment, a solution was prepared by mixing 12.98 mg of isopropanol plus 10.00 mL of an unknown containing some acetone, and diluting to 25.00 mL. Signal of 5.97 and 6.38 were observed for the acetone and isopropanol, respectively. In an earlier experiment, the Response Factor for acetone (analyte) to isopropanol (internal standard) was found to be 2.11. What is the concentration of acetone in the unknown solution?
Answer is around .576
I'm trying to use this equation: [X] = Ix/Is [S]/F but I how do I solve for [S] first?
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