. If you counted 20 colonies from a 0.01-ml inoculum of a 1:10 dilution of urine, how many organisms per milliliter of specimen would you report? Is this number significant?

2 answers

The inoculum is considered first which would be 20 X 100 / ml or 2000 / ml. Since you have diluted it again the concentration is higher by a factor of 10 or 10 X 2000 / ml = 20,000 / ml. Urine is not expected to be aseptic; however, it must be completely free of high-virulence microbial pathogens, and the total number of aerobic microorganisms per gram must be low.
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