If the corrected A260 of a DNA sample equals 1.65, then the concentration in that sample equals 1.65 ng DNA/μL.

Question

Question 4 options:
True
False

Answer 1

The statement is False.

The absorbance at 260 nm (A260) provides an estimate of nucleic acid concentration, but it's important to consider the specific extinction coefficient for DNA to convert A260 readings into concentration.

For double-stranded DNA, the general conversion factor is that an A260 of 1 corresponds to approximately 50 ng/µL. Therefore, an A260 reading of 1.65 would suggest a concentration of approximately:

1.65 * 50 ng/µL = 82.5 ng/µL, not 1.65 ng/µL.

So, the correct concentration in this context would be around 82.5 ng/µL.