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As an extension, CRISPR-Cas9 could be utilised on different genes that affect the pathophysiology of thalassemia, such as HBG1/2 and KLF1. Alteration of several different genes could have synergistic effects on foetal haemoglobin levels in thalassemia- (Talk a bit about how different genes can be used)
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Another potential extension could involve using CRISPR-Cas9 to target regulatory regions that influence the expression of multiple genes responsible for thalassemia, potentially leading to more comprehensive and effective treatments for the disease.