How to make 100 mL of 100mM of Tris HCl and 100 mL of 100mM of Tris Base. Follow the pH of the resulting combined solution as it is heated from 4 to 50 degrees Celsius.
I did not use the HH equation in this problem. I started with just converting to moles needed and then to grams. Then I was planning to add water to the mark. Since Tris is a base that's all I thought was needed.
For Tris HCl I was planning to follow the same protocol but later just to add HCl until the desired pH (dropwise). Do I have the right protocol? Also should I be using HH equation for this protocol or not?
1 answer
Either way is appropriate, but using a procedure for such preps is more reliable. See suggested procedure and example in answer to your next question.