During the light reactions of photosynthesis, water (H₂O) is split through a process known as photolysis. This process occurs in the thylakoid membranes of the chloroplasts in plants and is driven by light energy. Here's how it works in more detail:
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Light Absorption: Light energy is absorbed by pigment molecules in the thylakoid membranes, primarily chlorophyll. This energy excites electrons and initiates a series of photochemical reactions.
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Water Splitting: The energy from the excited electrons is used to power an enzyme complex called Photosystem II (PSII). This complex has a manganese cluster that facilitates the splitting of water molecules (H₂O) into hydrogen ions (H⁺), electrons (e⁻), and oxygen (O₂) gas. The overall reaction can be summarized as: \[ 2 H₂O \rightarrow 4 H⁺ + 4 e⁻ + O₂ \]
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Electron Transport Chain: The electrons released from the splitting of water replace the electrons that were lost by chlorophyll when it became excited. These electrons then enter the electron transport chain, where they move through a series of proteins and help generate an electrochemical gradient, contributing to the production of ATP and NADPH, which are essential for the subsequent dark reactions (Calvin cycle).
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Oxygen Release: The oxygen produced from this process is released as a byproduct into the atmosphere.
In summary, during the light reactions, water is split to provide electrons and protons that are essential for the formation of ATP and NADPH while releasing oxygen as a byproduct.