How does pH affect the activity of catalase? Consider both high and low pH, and explain your observations by discussing the effect of pH on protein structure.
I cannot find anywhere in my notes, or text book, or even on the internet how pH affects catalase. I know that with pH, as with temperature, catalase has an optimal pH at which it functions. But other than that I have no idea how to answer the question! Please help!
3 answers
Remember catalase is a type of protein. When it is exposed to extreme temperatures, it is going to denature - or change its shape (especially in active site) and would not be able to bind to its substrate.
Just like temperature increase can denature proteins, sharp decrease or increase in pH level can do the same.
If you look up "low pH denature a protein" in google, you can find this information. (that's how I found it)