To calculate the adjusted amount of energy that is available to organisms that are one trophic level above producers, you would use:
Gross primary productivity minus amount of organic material used in respiration.
This is because the gross primary productivity (GPP) represents the total energy captured by producers, while the amount used in respiration is the energy that is lost and not available for the next trophic level. Therefore, to find the energy available to the next trophic level, you need to account for the energy used by producers in respiration.
So, the correct response is:
gross primary productivity minus amount of organic material used in respiration.