To calculate the adjusted amount of energy available to organisms that are one trophic level above producers, you would focus on the energy that remains after accounting for the energy used by producers for respiration.
The correct choice is:
gross primary productivity minus amount of organic material used in respiration.
This is because Gross Primary Productivity (GPP) represents the total amount of energy captured by photosynthesis, while the energy used in respiration is subtracted from this to determine what is actually available for growth and reproduction, known as Net Primary Productivity (NPP). The energy available to the next trophic level (herbivores or primary consumers) is thus derived from the NPP of the producers.