The colonists’ rejection of the Proclamation of 1763 primarily led to the option "More and more settlers moved onto Indigenous lands."
The Proclamation was intended to stabilize relations between British settlers and Indigenous peoples by prohibiting colonial expansion west of the Appalachian Mountains. However, many colonists disregarded this order, leading to an influx of settlers encroaching on Indigenous territories. This resulted in increased conflicts, loss of land, and significant negative impacts on Indigenous communities as their resources and territories were systematically taken over. While the other options describe significant historical contexts, the primary and direct effect of the colonists' rejection was the continued encroachment on Indigenous lands.