How did Spain's refusal to destroy Negro Fort affect Florida? A. it showed that spain did not view General Jackson as a significant threat. B. it led to General Jackson's troops' first invasion of the territory. C. it gave the Seminoles an empowered stance against the United States. D. it prompted General Jackson to return to Washington DC.

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The correct answer is B. it led to General Jackson's troops' first invasion of the territory.

Spain's refusal to destroy Negro Fort, which was a stronghold for runaway slaves and Native Americans, contributed to tensions that prompted General Andrew Jackson to lead military actions in Florida. This was part of a broader context of conflicts involving U.S. interests and the Seminole tribe in the region, ultimately leading to the First Seminole War.

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