How can you determine the concentration of a sample vegetable using laboratory investigation?

1 answer

One common method for determining the concentration of a sample vegetable in a laboratory investigation is by using spectrophotometry. This involves measuring the absorption of light by the sample at specific wavelengths.

First, a solution is prepared by extracting the desired compounds from the vegetable sample. This could involve techniques such as solvent extraction or steam distillation. Then, the solution is placed in a spectrophotometer, which will measure the amount of light absorbed by the compounds present in the sample.

By comparing the absorption of the sample to a standard curve generated from known concentrations of the compound, the concentration of the compound in the vegetable sample can be determined.

Other methods for determining the concentration of compounds in a vegetable sample include chromatography, mass spectrometry, and various chemical tests. The choice of method will depend on the specific compound of interest and the sensitivity required for the analysis.