To determine the pH at which all of the amylase becomes denatured, you would need to explore various pH levels within the provided options. Amylase, like many enzymes, has an optimal pH range for activity, which typically falls around neutral (pH 7). However, outside of its optimal pH range, particularly in either more acidic (below 7) or more basic (above 7) conditions, amylase can become denatured.
Based on general knowledge regarding amylase's stability, it is likely that you will find denaturation occurring at pH levels significantly higher than 7 or lower than 7. Given the choices provided, the most reasonable pH levels to explore for denaturation would be:
- 5.5 and 8.5: It is plausible that amylase could be denatured at these pH levels, as both are outside of the neutral pH range.
So, your answer would be 5.5 and 8.5 as the pH levels at which all of the amylase becomes denatured.