Hi, I have a question on HPLC (high performance liquid chromatography).

Question

The procedure says to use a
4.6 x 150mm x 3um column.
The relative retention time of Impurity B with reference to the main peak is about 0.5min.
If I use a 4.6 x 100mm x 2.7um column, will the relative retention time of Impurity B with reference to the main peak still be about 0.5min?

Answer 1

See your post above and the response by Bob Pursley.