Hi guys,

Question

I don't quite understand why a lower Km value means more effective binding?

Sincerely,
Sandy

Answer 1

http://www.worthington-biochem.com/introbiochem/substrateconc.html

If the Michaelis factor is lower, more of the enzymes have bound in to a substrate-enzyme complex, resulting in a higher velocity of reaction production. The max km of course is at 1/2 the max velocity (Calculus is a wonderful tool to figure that out). So lower km means higher velocity than 1/2, and more are bound.