Gram positive and gram negative

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organisms are two different types of bacteria that can be differentiated by a laboratory staining technique called the Gram stain. The key difference between these two types of bacteria is in their cell wall structure.

Gram positive bacteria have a thick peptidoglycan layer in their cell wall that retains the crystal violet stain used in the Gram staining process, making them appear dark purple under the microscope. They also have teichoic acids in their cell wall that help maintain its structure. Some examples of Gram positive bacteria include Staphylococcus aureus, Streptococcus pneumoniae, and Bacillus subtilis.

Gram negative bacteria, on the other hand, have a thinner peptidoglycan layer and an outer membrane that contains lipopolysaccharides and porins. These components make the cell wall more complex and resistant to certain antibiotics. Gram negative bacteria do not retain the crystal violet stain and instead appear pink or red under the microscope after being counterstained with safranin. Some examples of Gram negative bacteria include Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa.

Knowing whether a bacterium is gram positive or gram negative is important in determining appropriate treatment options, as different antibiotics target specific components of the cell wall.