Glycerol 3-phosphate dehydrogenase catalyzes the reversible reaction:
G3P + NAD+ --> NADH + H+ + DHAP
If in the cell, the concentrations of G3P, NAD+, NADH, and DHAP are 2 mM, 4 mM, 6 mM, and 8 mM respectively. Determine if the reaction spontaneous as written?
DHAP + 2e– + 2H+ -->G3P DE'° = –0.29 V
NAD+ + H+ + 2e– --> NADH DE'° = –0.32 V