To identify a substitution mutation, we need to find a sequence where one nucleotide has been replaced by a different nucleotide compared to the original sequence provided:
Original Sequence:
C G T T A C T G C A A T G C A A T G A C G T T A
We can analyze the response options by comparing each one to the original sequence. A substitution mutation will involve one specific nucleotide changing to another while keeping the rest of the sequence unchanged at that position.
Let’s look at each option:
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C G T C T A C T G C A A T G C A G A T G A C G T T A
- Here, the first "T" is substituted by "C". This is a mutation.
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C G T C T A C T G C A A T G C A G A T G A C G T T A
- Same as above, the first "T" is substituted by "C".
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C G T A C T G C A A T G C A T G A C G T T A
- Here, the second "T" has been substituted by "A".
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C G T T A C T G C A A T G C A T G A C G T T A
- Here, the first sequence is identical to the original, no substitutions.
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C G T T A C T G C A A T G C A A T G A C G T T A
- Here, the same sequence as original still holds. No substitutions.
From these analyses, the sequences with substitution mutations are:
- C G T C T A C T G C A A T G C A G A T G A C G T T A
- C G T A C T G C A A T G C A T G A C G T T A
Therefore, the sequences showing substition mutations are the first and third options. If only one is allowed, either option can be correct based on which substitution example the question is focusing on.
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