The correct response to the question about the difference between gene regulation in prokaryotes and eukaryotes is:
Prokaryotes do not store their genes in a nucleus like eukaryotes do.
This is a fundamental difference, as prokaryotic cells (bacteria and archaea) have no membrane-bound nucleus and their genetic material is located in the cytoplasm, while eukaryotic cells (plants, animals, fungi, etc.) have their DNA enclosed within a nucleus.