To determine the pH at which amylase becomes denatured, we typically know that amylase has an optimal pH range that usually falls between 6.0 and 7.5. Outside of this range, particularly at more acidic or more basic pH levels, the enzyme may begin to denature.
From the options provided, the expected pH ranges for denaturation would be:
- 6.0 and 7.5 (neutral, within optimal range)
- 5.5 and 7.5 (slightly acidic, close to neutral)
- 5.5 and 8.5 (a bit more acidic and slightly basic)
- 6.5 and 7.5 (within optimal range)
Given the information about amylase, it is likely that its denaturation occurs at pH levels below 5.5 and above 7.5. Therefore, the option that suggests more acidic and more basic endpoints would be the best candidate for the pH range resulting in denaturation of amylase.
So the best answer would be: 5.5 and 8.5