To increase an enzyme's activity, you would typically consider the following changes:
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Raising the temperature: This can increase enzyme activity up to a certain point (the optimum temperature), as higher temperatures generally increase the kinetic energy of molecules, leading to more frequent enzyme-substrate interactions. However, excessively high temperatures can denature the enzyme and decrease activity.
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Increasing the enzyme’s concentration: This usually increases the rate of reaction, assuming there is a sufficient amount of substrate available. More enzyme molecules lead to more active sites for substrate binding.
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Decreasing the enzyme’s concentration: This would decrease enzyme activity, as there would be fewer enzyme molecules available to catalyze the reaction.
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Lowering the temperature: This typically decreases enzymatic activity, as it reduces kinetic energy and the likelihood of enzyme-substrate collisions.
Given these points, the changes that would increase an enzyme's activity are:
- Raising the temperature (to an optimal level).
- Increasing the enzyme’s concentration.
So, both raising the temperature (to a certain extent) and increasing the enzyme's concentration would increase enzyme activity.