EcoRI recognizes the palindrome sequence GAATTC and breaks the phosphodiester bonds between the G and A (G*A) on both strands. Draw a diagram to demonstrate what the sticky-ends would look like.

Answer 1

5'-G A A TTC-3'
3'-C T T AAG-5'

After EcoRI digestion, the resulting sticky ends would be:
5'-G AATTC-3'
3'-CTTAA G-5'

Note the overhangs on both strands, indicated by the single-stranded regions at the end of each strand. These can base-pair with complementary overhangs on other fragments with the same Sticky end sequences.