e) In performing an immunological procedure, you add 2 mL of serum to 8 mL of saline. Two 1:2 serial dilutions are then made. What is the dilution of serum contained in the final tube?

Answer 1

you diluted it first by a facator of 5 (2ml to 10 ml).
then you diluted it by factors of 3.

dilution: 5*3*3=45 times