The event most directly responsible for increased tensions between Byzantine and western Christians during the Crusades was:
C. Crusaders attacked Constantinople on their way to Egypt; the sack of Constantinople in 1204 during the Fourth Crusade worsened the relationship between the Orthodox and Roman Catholic Churches and weakened the Byzantine Empire.
This event had a profound impact on the relations between the two Christian branches, leading to lasting mistrust and animosity. The Fourth Crusade, which was originally intended to recapture the Holy Land, instead culminated in the sacking of Constantinople, further deteriorating the already strained relationship between the Eastern Orthodox Church and the Roman Catholic Church.