During lab, you are provided with a stock antibody solution (Ab) that has a concentration of 400 μg/μl. For lab, it is necessary make the following dilutions (Fill in the blanks):

5μL of Stock Ab + 195μL of buffer to make a 1:____ dilution at _______μg/μL

20μL of 1:100 stock + 60μL of buffer to make a 1:____dilution at ______μg/μL