Reading frame refers to the specific way in which the nucleotide sequence of DNA or RNA is read during gene expression, typically in groups of three nucleotides called codons. In a normal reading frame, the genetic code is read without interruption, resulting in the production of a functional protein. Frame shift mutation, on the other hand, is a type of genetic mutation that occurs when a nucleotide is deleted or inserted into the DNA sequence, causing a shift in the reading frame. This shift can alter the genetic code and result in the production of a non-functional protein.
Primer DNA is a short, artificially synthesized piece of DNA that serves as a starting point for DNA synthesis in the polymerase chain reaction (PCR). It is designed to hybridize with a specific region of template DNA and provide a free 3'-OH group for DNA polymerase to build off of. Template DNA, on the other hand, is the original strand of DNA that is being replicated or transcribed. It serves as a template for the complementary base pairing of nucleotides during DNA synthesis or mRNA transcription.
Difference between reading frame and frame shift mutation. Also primer DNA and template DNA
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