Devise a plan to carry out the following growth curve:
Seed cells on day -1.
Transfer cells to the test media on day 0.
From day 0, plan to carry out your growth curve for 10 days.
During this time, the cells are to be fed three times (excluding the day 0 medium change), and enumerated 4 times.
The test media conditions are medium that contains 4% serum and medium that contains 12% serum.
How many culture vessels do you need?
How much media do you need? This should include all procedures.
When are you going to do what?