The process of gene expression can be summarized in the following steps, in their correct order:
- Transcription: The DNA sequence of a gene is copied into messenger RNA (mRNA). This occurs in the nucleus of eukaryotic cells.
- RNA Processing: The primary mRNA transcript undergoes processing, which includes the addition of a 5' cap, polyadenylation (adding a poly-A tail), and splicing (removal of introns).
- Translation: The processed mRNA is transported to the ribosome, where it is translated into a protein. Transfer RNA (tRNA) molecules bring the appropriate amino acids to the ribosome based on the codon sequence of the mRNA.
- Post-Translational Modifications: After translation, the protein may undergo further modifications, such as phosphorylation or glycosylation, to become fully functional.
In summary, the order is: Transcription → RNA Processing → Translation → Post-Translational Modifications.