To determine which species is most closely related to species 4, we would need to see the specific phylogenetic tree you mentioned. However, without seeing the tree, I can't definitively provide the answer.
In general, you can identify the closest relatives on a phylogenetic tree by looking at which species share a more recent common ancestor with the species in question. If species 4 shares a branch with another species that is closer to it than to the others, that species would be the closest relative.
If you can provide more details or describe the tree, I can help guide you more accurately. But based on your given options, the correct answer would be either 1, 3, or 5, depending on the tree's structure.