In "Twelfth Song of Thunder," the most important themes aligned with the Navajo people include a deep respect for nature and an appreciation of life. The song emphasizes the interconnectedness of all living things and the spiritual significance of nature in the Navajo worldview.
Therefore, while all of your options hold significance within Navajo culture, 1. respect for nature and 3. appreciation of life particularly resonate with the themes presented in the song.