are the well in gel electrophoresis negative or positive?

1 answer

In gel electrophoresis, the wells are typically considered to be at a negative potential when using agarose or polyacrylamide gels. This is because the gel is usually submerged in a buffer solution that contains charged ions. When an electric field is applied across the gel, the negatively charged DNA or RNA molecules migrate towards the positive electrode, which is located at the opposite end of the gel from the wells. As a result, the wells themselves, where the samples are loaded, are associated with a negative charge.