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) Anthranilate fluoresces blue under ultraviolet light. Under normal conditions, the levels of anthranilate in bacterial cells are low and the cells do not visibly fluoresce. However, if anthranilate accumulates because a relevant enzyme is defective or absent, the cells will fluoresce.
The table below lists several enzymes in the tryptophan biosynthetic pathway. Each row corresponds to a different bacterial strain. A “+” in the table indicates that the gene for that enzyme is functional in that strain. A “-“ in the table indicates that the gene for that enzyme is non-functional in that strain. For each strain, indicate whether or not you would expect to see blue fluorescence under ultraviolet light.
The table below lists several enzymes in the tryptophan biosynthetic pathway. Each row corresponds to a different bacterial strain. A “+” in the table indicates that the gene for that enzyme is functional in that strain. A “-“ in the table indicates that the gene for that enzyme is non-functional in that strain. For each strain, indicate whether or not you would expect to see blue fluorescence under ultraviolet light.
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