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Question

by Tasheena Orelue - Tuesday, 3 December 2024, 1:31 PM
Number of replies: 0
I have a question that I know seems simple, and maybe I am overthinking this. However it is confusing me. When doing testing of antibiotics and setting up the control disk..... is the control disk meant to have just like solution on it so that it can be monitored to ensure that the outcome of the other tests arent due to a faulty disk and actually is the result of the antibiotic they obtain or is the control disk meant to be dosed with something on it also, or left clean? I'm so lost when it comes to the cotrol disk and how to set it up and what it is meant to do. HELP PLEASE! lol.

Answer 1

The control disk in antibiotic testing serves an important role in ensuring the accuracy and reliability of the results. Typically, the control disk is not left clean; instead, it contains a known concentration of an antibiotic that is used as a standard to compare against the test disks with the antibiotics being evaluated.

The purpose of the control disk is to help verify that the testing procedure and the media used are functioning correctly. By including a control disk, you can determine if the antibiotic susceptibility results for your test organisms are valid. If the control disk produces expected results (i.e., a clear zone of inhibition at the right size for the known antibiotic), you can be more confident that the results from the other disks reflect the true antibiotic activity against the bacteria being tested.

In summary, when setting up the control disk, it should be treated in the same way as the test disks but should contain an antibiotic with a well-established expected outcome for that particular pathogen. This way, you can confirm that any variation in results from your test disks is due to the antibiotics being tested rather than an error in the procedure itself. If you have any further questions or need clarification, feel free to ask!