The most important aspect missing from Elaina's scientific investigation is A testable hypothesis naming independent and dependent variables.
While she has a general idea of her hypothesis regarding the effect of music tempo on pulse rate, she needs to explicitly state a clear hypothesis that defines the independent variable (the tempo of the music) and the dependent variable (the pulse rate).
The other options mentioned might be part of a comprehensive investigation, but the clear formulation of a testable hypothesis is foundational to guiding the research and ensuring it is scientifically sound.