After an enzyme is mixed with its substrate, the amount of product formed is determined at 10-sec intervals for 1 min.

Data:
0 secs. = 0.0 product formed (mg)
10 secs = .25 product formed (mg)
20 secs = .50 product formed (mg)
30 secs = .70 product formed (mg)
40 secs = .80 product formed (mg)
50 secs = .85 product formed (mg)
60 secs = .85 product formed (mg)

First of all how do i find the intial rate of the enzymatic reaction? Than, if i was suppose to find the rate after 50 secs, what would it be?

What would happen if i heated the enzyme for 10 mins in 100 degrees celsius before repeating the experiment and why does this happen?

Altering the substrate concentration could effect the rate of reaction?

Changing the ph would effect the rate of the reaction as well?

1 answer

To find the initial rate of the enzymatic reaction, you would need to calculate the change in product formed over the change in time. In this case, the initial rate of the reaction would be 0.25 mg/10 secs.

The rate after 50 secs would be 0.85 mg/50 secs.

If you heated the enzyme for 10 mins in 100 degrees Celsius before repeating the experiment, the enzyme would likely denature, meaning it would lose its shape and become inactive. This would result in a decrease in the rate of the reaction.

Altering the substrate concentration could affect the rate of reaction. Increasing the substrate concentration would increase the rate of reaction, while decreasing the substrate concentration would decrease the rate of reaction.

Changing the pH could also affect the rate of the reaction. Enzymes typically have an optimal pH at which they work best, and changing the pH away from this optimal value could decrease the rate of the reaction.
Similar Questions
    1. answers icon 1 answer
  1. Please help, with the following question:"When an enzyme reaches its saturation point,..." A) the amount of substrate for the
    1. answers icon 1 answer
  2. Identify the following in the FIRST HALF of the reaction above.* 3 points Starch Maltose Amylase Maltase Glucose Product
    1. answers icon 1 answer
    1. answers icon 1 answer
more similar questions