To rotate a point \((x, y)\) 90° clockwise around the origin, the new coordinates become \((y, -x)\).
Let's apply this to each endpoint of the line segment.
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Rotating the first endpoint (2, -1): \[ (x, y) = (2, -1) \quad \Rightarrow \quad (y, -x) = (-1, -2) \]
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Rotating the second endpoint (5, -4): \[ (x, y) = (5, -4) \quad \Rightarrow \quad (y, -x) = (-4, -5) \]
So after rotating the line segment 90° clockwise, the new endpoints are \((-1, -2)\) and \((-4, -5)\).
Therefore, the correct response is: \[ (-1, -2) \text{ and } (-4, -5) \]