To find the concentration of protein in the unknown sample, we can use the equation for Beer's Law:
A = εcl
Where A is the absorbance, ε is the molar absorptivity (also known as the slope of the calibration curve), c is the concentration of the protein, and l is the path length of the cuvette (which is typically 1 cm).
Plugging in the values we have:
0.345 = (0.433) * c * 1
To isolate the concentration, divide both sides of the equation by 0.433:
0.345 / 0.433 = c
Calculating this, we find that the concentration of protein in the sample is approximately 0.796 mg/mL.