Asked by OR
Can someone help me calculate protein concentration using the Bradford Assay?
If you could show me how to do tube 2, that'd be great.
Tube 1: 0 ul Protein Stock Solution, 800 ul Water, 0 ug Protein, Absorbance @595nm = 0
Tube 2: 10 ul Protein Stock Solution, 790 ul Water, 1.4 ug Protein, Absorbance @595nm = .068
Tube 3: 20 ul Protein Stock Solution, 780 ul Water, 2.8 ug Protein, Absorbance @595nm = .144
Tube 4: 40 ul Protein Stock Solution, 760 ul Water, 5.6 ug Protein, Absorbance @595nm = .256
Tube 5: 60 ul Protein Stock Solution, 740 ul Water, 8.4 ug Protein, Absorbance @595nm = .361
Tube 6: 80 ul Protein Stock Solution, 720 ul Water, 11.2 ug Protein, Absorbance @595nm = .464
I have to determine the protein concentration and plot it against the absorbance.
If you could show me how to do tube 2, that'd be great.
Tube 1: 0 ul Protein Stock Solution, 800 ul Water, 0 ug Protein, Absorbance @595nm = 0
Tube 2: 10 ul Protein Stock Solution, 790 ul Water, 1.4 ug Protein, Absorbance @595nm = .068
Tube 3: 20 ul Protein Stock Solution, 780 ul Water, 2.8 ug Protein, Absorbance @595nm = .144
Tube 4: 40 ul Protein Stock Solution, 760 ul Water, 5.6 ug Protein, Absorbance @595nm = .256
Tube 5: 60 ul Protein Stock Solution, 740 ul Water, 8.4 ug Protein, Absorbance @595nm = .361
Tube 6: 80 ul Protein Stock Solution, 720 ul Water, 11.2 ug Protein, Absorbance @595nm = .464
I have to determine the protein concentration and plot it against the absorbance.
Answers
There are no human answers yet.
There are no AI answers yet. The ability to request AI answers is coming soon!
Submit Your Answer
We prioritize human answers over AI answers.
If you are human, and you can answer this question, please submit your answer.