Why is it difficult to reestablish a species permanently into its natural environment once the total number of a species drop below a population of 500?

Thank you for your help.

2 answers

The genetic variability is limited?
Usually when a species population gets to a point where it is that endangered there is probably no way to re-establish it. There is probably severe problems in either their genes, habitat, disease has happened that is incurable, or a loss in diversity among the animal species.
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